Vivaspin 20, MWCO 3 - 100 kDa

Pack size 12

Vivaspin 20 contains 12 disposable Vivaspin 20 ultrafiltration devices with either 3,000, 5,000, 10,000, 30,000, 50,000 or 100,000 MWCO PES. Vivaspin spin columns are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.

Vivaspin 20 can handle samples up to 20 ml.

Vivaspin 20 features twin vertical membranes for unparalleled speed.

• High recovery rates – Up to 30-fold concentration of the sample can be achieved, with recovery of the target molecule typically exceeding 95%.
• Sample protection – the patented Vivaspin dead-stop technology ensures samples cannot be concentrated to dryness and enables direct concentrate recovery.
• Ease-of-use – one-step sample concentration in a single tube, for minimal sample handling and reduced sample loss.
• pH compatibility – for a broad range of pH 1 to 9.
• High flow rates – and minimal membrane blockage through the vertical polyethersulfone (PES) membrane design.
• Minimal protein adsorption – thanks to the Vivaspin polycarbonate tube design.

Spin Column Protein Concentration – How Does it Work?

Vivaspin sample concentrators cater to scientists working with sample volumes ranging from 100 μl to 20 ml, with a range of MWCO values from Mr 3 000 to 100 000.

An upper compartment contains sample and the lower compartment separated by a semipermeable membrane. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.

Tip: To maximize recovery of your sample, select a MWCO at least 50% smaller than the molecular size of the target molecule.